Publications

  1. Kimura R, Nakata M, Funabiki Y, Suzuki S, Awaya T, Murai T, Hagiwara M. (2019) An epigenetic biomarker for adult high-functioning autism spectrum disorder. Sci Rep. 9(1):13662.
  2. Nomura T, Sumi E, Egawa G, Nakajima S, Toichi E, Uozumi R, Tada H, Nakagawa T, Hagiwara M, Kabashima K. (2019) The efficacy of a cyclin dependent kinase 9 (CDK9) inhibitor, FIT039, on verruca vulgaris: study protocol for a randomized controlled trial. Trials. 20(1):489.
  3. Hosokawa M, Takeuchi A, Tanihata J, Iida K, Takeda S, Hagiwara M. (2019) Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy. iScience. 13:229-242.
  4. Ichisima J, Suzuki NM, Samata B, Awaya T, Takahashi J, Hagiwara M, Nakahata T, Saito MK. (2019) Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model. J Hum Genet. doi: 10.1038/s10038-019-0574-8.
  5. Korogi Y, Gotoh S, Ikeo S, Yamamoto Y, Sone N, Tamai K, Konishi S, Nagasaki T, Matsumoto H, Ito I, Chen-Yoshikawa TF, Date H, Hagiwara M, Asaka I, Hotta A, Mishima M, Hirai T. (2019) In Vitro Disease Modeling of Hermansky-Pudlak Syndrome Type 2 Using Human Induced Pluripotent Stem Cell-Derived Alveolar Organoids. Stem Cell Reports. 12(3):431-440.
  6. Kimura R, Swarup V, Tomiwa K, Gandal MJ, Parikshak NN, Funabiki Y, Nakata M, Awaya T, Kato T, Iida K, Okazaki S, Matsushima K, Kato T, Murai T, Heike T, Geschwind DH, Hagiwara M. (2019) Integrative network analysis reveals biological pathways associated with Williams syndrome. J Child Psychol Psychiatry. 60(5):585-598. doi.org/10.1111/jcpp.12999.
  7. Boisson B, Honda Y, Ajiro M, Bustamante J, Bendavid M, Gennery AR, Kawasaki Y, Ichishima J, Osawa M, Nihira H, Shiba T, Tanaka T, Chrabieh M, Bigio B, Hur H, Itan Y, Liang Y, Okada S, Izawa K, Nishikomori R, Ohara O, Heike T, Abel L, Puel A, Saito MK, Casanova JL, Hagiwara M, Yasumi T. (2018) Rescue of recurrent deep intronic mutation underlying cell type-dependent quantitative NEMO deficiency. J Clin Invest. 129(2):583-597.
  8. Sumi E, Nomura T, Asada R, Uozumi R, Tada H, Amino Y, Sawada T, Yonezawa A, Hagiwara M, and Kabashima K. (2018) Safety and Plasma Concentrations of a Cyclin-dependent Kinase 9 (CDK9) Inhibitor, FIT039, Administered by a Single Adhesive Skin Patch Applied on Normal Skin and Cutaneous Warts. Clin Drug Investig. 39(1):55-61.
  9. Kimura R, Ishii Y, Tomiwa K, Awaya T, Nakata M, Kato T, Okazaki S, Heike T, and Hagiwara M. (2018) Williams-Beuren Syndrome as a Potential Risk Factor for Burkitt Lymphoma. Front Genet. 9:368.
  10. Fuji H, Ohmae S, Noma N, Takeiri M, Yasutomi H, Izumi K, Ito M, Toyomoto M, Iwaki S, Takemoto K, Seo S, Taura K, Hida S, Aoyama M, Ishihama Y, Hagiwara M, Takeda N, Hatano E, Iwaisako K, Uemoto S, and Asagiri M. (2018) Necrostatin-7 suppresses RANK-NFATc1 signaling and attenuates macrophage to osteoclast differentiation. Biochem Biophys Res Commun. 503(2):544-549.
  11. Takeuchi A, Iida K, Tsubota T, Hosokawa M, Denawa M, Brown JB, Ninomiya K, Ito M, Kimura H, Abe T, Kiyonari H, Ohno K, and Hagiwara M (2018) Loss of Sfpq causes long-gene transcriptopathy in the brain. Cell Reports. 23(5):1326-1341.
  12. Ajiro M, Sakai H, Onogi H, Yamamoto M, Sumi E, Sawada T, Nomura T, Kabashima K, Hosoya T, and Hagiwara M (2018) CDK9 Inhibitor FIT-039 Suppresses Viral Oncogenes E6 and E7 and Has a Therapeutic Effect on HPV-Induced Neoplasia. Clin Cancer Res. 24(18):4518-4528.
  13. Yamada S, Nagai M, and Hagiwara M. (2018) The 40th Anniversary of the Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine. Anat Rec (Hoboken). 301(6):947-950.
  14. Yoshida S, Kanno K, Kii I, Misawa Y, Hagiwara M and Hosoya T (2018) Convergent synthesis of trifunctional molecules by three sequential azido-type-selective cycloadditions. Chem Comm 54(30):3705-3708.
  15. Wang DO, Ninomiya K, Mori C, Koyama A, Haan M, Kitabatake M, Hagiwara M, Chida K, Takahashi SI, Ohno M, and Kataoka N. (2017) Transport Granules Bound with Nuclear Cap Binding Protein and Exon Junction Complex Are Associated with Microtubules and Spatially Separated from eIF4E Granules and P Bodies in Human Neuronal Processes. Front Mol Biosci. 4:93.
  16. Nakano-Kobayashi A, Awaya T, Kii I, Sumida Y, Okuno Y, Yoshida S, Sumida T, Inoue H, Hosoya T, and Hagiwara M. (2017) Prenatal neurogenesis induction therapy normalizes brain structure and function in Down syndrome mice. Proc Natl Acad Sci USA. 114(38):10268-10273.
  17. Matsumoto S, Iida K, Murata A, Denawa M, Hagiwara M, and Nakatani K. (2017) Synthetic ligand promotes gene expression by affecting GC sequence in promoter. Bioorg Med Chem Lett. 27(15):3391-3394.
  18. Ohe K, Yoshida M, Nakano-Kobayashi A, Hosokawa M, Sako Y, Sakuma M, Okuno Y, Usui T, Ninomiya K, Nojima T, Kataoka N, and Hagiwara M. (2017) RBM24 promotes U1 snRNP recognition of the mutated 5′ splice site in the IKBKAP gene of familial dysautonomia. RNA 23(9):1393-1403.
  19. Ohta H, Kurimoto K, Okamoto I, Nakamura T, Yabuta Y, Miyauchi H, Yamamoto T, Okuno Y, and Hagiwara M, Shirane K, Sasaki H, and Saitou M. (2017) In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate. EMBO J. 36(13):1888-1907.
  20. Kawaguchi K, Suzuki E, Nishie M, Kii I, Kataoka TR, Hirata M, Inoue M, Pu F, Iwaisako K, Tsuda M, Yamaguchi A, Haga H, Hagiwara M, and Toi M. (2017) Downregulation of neuropilin-1 on macrophages modulates antibody-mediated tumoricidal activity. Cancer Immunol Immunother. 66(9):1131-1142.
  21. SakoY, Ninomiya K, Okuno Y, Toyomoto M, Nishida A, KoikeY, Ohe K, Kii I, Yoshida S, Hashimoto N, Hosoya T, Matsuo M, and Hagiwara M (2017) Development of an orally available inhibitor of CLK1 for skipping a mutated dystrophin exon in Duchenne muscular dystrophy. Sci Rep. 7:46126.
  22. Ohmae S, Noma N, Toyomoto M, Shinohara M, Takeiri M, Fuji H, Takemoto K, Iwaisako K, Fujita T, Takeda N, Kawatani M, Aoyama M, Hagiwara M, Ishihama Y, and Asagiri M. (2017) Actin-binding protein coronin 1A controls osteoclastic bone resorption by regulating lysosomal secretion of cathepsin K. Sci Rep. 7:41710.
  23. Nguyen BN, Okuno Y, Ajiro M, Iida K, Denawa, Yamamoto M, Sakamoto N, Kagechika H, and Hagiwara M. (2016) Retinoid derivative Tp80 exhibits anti-hepatitis C virus activity through restoration of GI-GPx expression. J Med Virol. 89(7):1224-1234.
  24. Candeias MM, Hagiwara M, and Matsuda M. (2016) Cancer-specific mutations in p53 induce the translation of Δ160p53 promoting tumorigenesis. EMBO Rep. 17(11):1542-1551.
  25. Tanaka T, Okuyama-Dobashi K, Murakami S, Chen W, Okamoto T, Ueda K, Hosoya T, Matsuura Y, Ryo A, Tanaka Y, Hagiwara M, and Moriishi K. (2016) Inhibitory effect of CDK9 inhibitor FIT-039 on hepatitis B virus propagation. Antiviral Research 133:156-164.
  26. Kii I, Sumida Y, Goto T, Sonamoto R, Okuno Y, Yoshida S, Kato-Sumida T, Koike Y, Abe M, Nonaka Y, Ikura T, Ito N, Shibuya H, Hosoya T, and Hagiwara M. (2016) Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun. 7:11391.
  27. Sakuma M, Iida K, and Hagiwara M. Deciphering targeting rules of splicing modulator compounds: case of TG003. BMC Mol Biol. (2015) 16:16.
  28. Okamoto M, Hidaka A, Toyama M, Hosoya T, Yamamoto M, Hagiwara M, and Baba M (2015) Selective inhibition of HIV-1 replication by the CDK9 inhibitor FIT-039. Antiviral Res. 123, 1-4.
  29. Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, Kii I, Nishiyama T, Kakita A, Takeda H, Takeuchi A, Arai Y, Ito I, Komura H, Hirao H, Satomura K, Inoue M, Muramatsu S, Matsui K, Tada M, Sato M, Saijo E, Shigemitsu Y, Sakai S, Umetsu Y, Goda N, Takino N, Takahashi H, Hagiwara M, Sawasaki T, Iwasaki G, Nakamura Y, Nabeshima Y, Teplow DB, and Hoshi M. (2015) Na,K-ATPaseα3 Is a Death Target of Alzheimer Patient Amyloid-β Assembly. Proc Natl Acad Sci USA. 112(32):E4465-4474.
  30. Sonamoto R, Kii I, Koike Y, Sumida Y, Kato-Sumida T, Okuno Y, Hosoya T, and Hagiwara M. (2015) Identification of a DYRK1A Inhibitor that Induces Degradation of the Target Kinase using Co-chaperone CDC37 fused with Luciferase nanoKAZ. Sci. Rep. 5:12728.
  31. Masaki S, Kii I, Sumida Y, Kato-Sumida, Ogawa Y, Ito N, Nakamura M, Sonamoto R, Kataoka N , Hosoya T, and Hagiwara M. (2015). Design and synthesis of a potent inhibitor of class 1 DYRK kinases as a suppressor of adipogenesis. Bioorg Med Chem. 23(15):4434-4441.
  32. Morooka S, Hoshina M, Kii I, Okabe T, Kojima H, Inoue N, Okuno Y, Denawa M, Suguru Yoshida, Fukuhara J, Ninomiya K, Ikura T, Furuya T, Nagano T, Noda K, Ishida S, Hosoya T, Ito N, Yoshimura N, and Hagiwara M. (2015) Identification of a Dual Inhibitor of SRPK1 and CK2 that Attenuates Pathological Angiogenesis of Macular Degeneration in Mice. Mol. Pharmacol. 88(2):316-325.
  33. Yoshida M, Kataoka N, Miyauchi K, Ohe K, Iida K, Yoshida S, Nojima T, Okuno Y, Onogi H, Usui T, Takauchi A, Hosoya T, Suzuki T, and Hagiwara M, (2015) Rectifier of aberrant mRNA splicing recovers tRNA modification in familial dysautonomia. Proc Natl Acad Sci USA. 112(9):2764-2769.
  34. Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Güntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, and Muto Y. (2014) RBFOX and SUP-12 sandwich a guanine base to form a stable complex and regulate tissue-specific splicing. Nat. Struct. Mol. Biol. 21(9):778-786.
  35. Yamamoto M, Onogi H, Kii I, Yoshida S, Iida K, Sakai H, Abe M, Tsubota T,
    Ito N, Hosoya T, and Hagiwara M. (2014) CDK9 inhibitor FIT-039 prevents replication of multiple DNA viruses. J Clin Invest. 124(8):3479–3488.
  36. Kurihara T, Sakurai E, Toyomoto M, Kii I, Kawamoto D, Asada T, Tanabe T, Yoshimura M, Hagiwara M, Miyata A. (2014) Alleviation of behavioral hypersensitivity in mouse models of inflammatory pain with two structurally different casein kinase 1 (CK1) inhibitors. Mol Pain. 10:17
  37. Gammons MV, Fedorov O, Ivison D, Du C, Clark T, Hopkins C, Hagiwara M, Dick AD, Cox R, Harper SJ, Hancox JC, Knapp S, Bates DO. (2013) Topical antiangiogenic SRPK1 inhibitors reduce choroidal neovascularization in rodent models of exudative AMD. Invest Ophthalmol Vis Sci. 54(9):6052-6062.
  38. Pozo N, Zahonero C, Fernández P, Liñares JM, Ayuso A, Hagiwara M, Pérez A, Ricoy JR, Hernández-Laín A, Sepúlveda JM, Sánchez-Gómez P. (2013) Inhibition of DYRK1A destabilizes EGFR and reduces EGFR-dependent glioblastoma growth. J Clin Invest. 123(6):2475-2487.
  39. Dong Z, Noda K, Kanda A, Fukuhara J, Ando R, Murata M, Saito W, Hagiwara M, Ishida S (2013) Specific inhibition of serine/arginine-rich protein kinase attenuates choroidal neovascularization. Mol Vis. 19:536-543
  40. Kuroyanagi H, Watanabe Y, Suzuki Y, and Hagiwara M. (2013) Position-dependent and neuron-specific splicing regulation by the CELF family RNA-binding protein UNC-75 in Caenorhabditis elegans. Nucleic. Acids Res. 41(7):4015-4025.
  41. Kuroyanagi H, Watanabe Y, and Hagiwara M. (2013) CELF family RNA-binding protein UNC-75 regulates two sets of mutually exclusive exons of the unc-32 gene in neuron-specific manners in Caenorhabditis elegans. PLoS Genet. 9(2):e1003337
  42. Kataoka N, Dobashi I, Hagiwara M, and Ohno M. (2013) hDbr1 is a nucleocytoplasmic shuttling protein with a protein phosphatase-like motif essential for debranching activity. Sci Rep 3, 1090.
  43. Ohno G, Ono K, Togo M, Watanabe Y, Ono S, Hagiwara M, Kuroyanagi H (2012) Muscle-specific splicing factors ASD-2 and SUP-12 cooperatively switch alternative pre-mRNA processing patterns of the ADF/cofilin gene in C. elegans. PLoS Genet 8(10):e1002991.
  44. Saitoh N, Sakamoto C, Hagiwara M, Agredano-Moreno LT, Jiménez-García LF, Nakao M. (2012) The distribution of phosphorylated SR proteins and alternative splicing are regulated by RANBP2. Mol Biol Cell. 23(6):1115-1128.
  45. Kusano-Kitazume A, Sakamoto N, Okuno Y, Sekine-Osajima Y, Nakagawa M, Kakinuma S, Kiyohashi K, Nitta S, Murakawa M, Azuma S, Nishimura-Sakurai Y, Hagiwara M, Watanabe M. (2012) Identification of novel N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication. Antimicrob Agents Chemother, 56(3):1315-1323.
  46. Amin EM, Hua J, Cheung MK, Ni L, Kase S, Ren-nel ES, Gammons M, Nowak DG, Saleem MA, Hagiwara M, Schumacher VA, Harper SJ, Hinton D, Bates DO, Ladomery MR (2011) WT1 mutants reveal SRPK1 to be a downstream angiogenesis target by altering VEGF splicing. Cancer Cell 20(6):768-780.
  47. Kataoka N, Diem MD, Yoshida M, 4, Hatai C, Dobashi I, Dreyfuss G, Hagiwara M, and Ohno M. (2011) Specific Y14 domains mediate its nucleo-cytoplasmic shuttling and association with spliced mRNA. Sci Rep 1:92.
  48. Ninomiya K, Kataoka N, and Hagiwara M. (2011) Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor. J Cell Biol. 195(1):27-40.
  49. Anwar A, Hosoya T, Leong KM, Onogi H, Okuno Y, Hiramatsu T, Koyama H, Suzuki M, Hagiwara M, Garcia-Blanco MA.(2011) The Kinase Inhibitor SFV785 Dislocates Dengue Virus Envelope Protein from the Replication Complex and Blocks Virus Assembly. PLoS One. 6(8):e23246.
  50. Debdab M, Carreaux F, Renault S, Soundararajan M, Fedorov O, Filippakopoulos P, Lozach O, Babault L, Tahtouh T, Baratte B, Ogawa Y, Hagiwara M, Eisenreich A, Rauch U, Knapp S, Meijer L, Bazureau JP. (2011) Leucettines, a class of potent inhibitors of cdc2-like kinases and dual specificity, tyrosine phosphorylation regulated kinases derived from the marine sponge leucettamine B: modulation of alternative pre-RNA splicing. J Med Chem. 54(12):4172-4186.
  51. Nishida A, Kataoka N, Takeshima Y, Yagi M, Awano, H, Ota, M, Itoh K, Hagiwara M, and Matsuo M (2011) Chemical treatment enhances skipping of a mutated exon in the dystrophin gene. Nature Commun 2, 308.
  52. Sims-Lucas, S., Cusack, B., Baust, J., Eswarakumar, V.P., Hagiwara M., Takeuchi, A., and Bates, C.M. (2011). Fgfr1 and the IIIc isoform of Fgfr2 play critical roles in the metanephric mesenchyme mediating early inductive events in kidney development. Dev Dyn 240(1):240-249.
  53. Ogawa Y, Nonaka Y, Goto T, Ohnishi E, Hiramatsu T, Kii I, Yoshida M , Ikura T , Onogi H , Shibuya H , Hosoya T, Ito N, and Hagiwara M (2010) Development of a novel selective inhibitor of the Down syndrome-related kinase Dyrk1A. Nature Commun. 1: 86,
  54. Karakama Y, Sakamoto N, Itsui Y, Nakagawa M, Tasaka-Fujita M, Nishimura-Sakurai Y, Kakinuma S, Oooka M, Azuma S, Tsuchiya K, Onogi H, Hagiwara M, and Watanabe M. (2010) Inhibition of hepatitis C virus replication by a specific inhibitor of serine-arginine-rich protein kinase. Antimicrob Agents Chemother. 54(8):3179-3186.
  55. Kii I, Shiraishi A, Hiramatsu T, Matsushita T, Uekusa H, Yoshida S, Yamamoto M, Kudo A, Hagiwara M, and Hosoya T. (2010) Strain-promoted double-click reaction for chemical modification of azido-biomolecules. Org Biomol Chem. 8(18):4051-4055.
  56. Takeuchi A, Hosokawa M, Nojima T, Hagiwara M (2010) Splicing reporter mice revealed the evolutionally conserved switching mechanism of tissue-specific alternative splicing. PLoS One 5(6):e10946.
  57. Kuroyanagi H, Ohno G, Sakane, H, Maruoka, H, and Hagiwara M (2010) Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans. Nature Protoc. 5(9):1495-1517
  58. Nowak DG, Amin EM, Rennel ES, Hoareau-Aveilla C, Gammons M, Damodoran G, Hagiwara M, Harper SJ, Woolard J, Ladomery MR, and Bates DO. (2010) Regulation of vascular endothelial growth factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: a novel therapeutic strategy for angiogenesis. J. Biol. Chem. 285(8):5532-5540.
  59. Nojima T, Oshiro-Ideue T, Nakanoya H, Kawamura H, Morimoto M, Kawaguchi Y, Kataoka N and Hagiwara M (2009) Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing. Nucleic. Acids Res. 37(19):6515-6527.
  60. Jiang K, Patel NA, Watson JE, Apostolatos H, Kleiman E, Hanson O, Hagiwara M, Cooper DR. (2009) Akt2 regulation of Cdc2-like kinases (Clk/Sty), serine/arginine-rich (SR) protein phosphorylation, and insulin-induced alternative splicing of PKCβII mRNA. Endocrinology, 150(5):2087-2097.
  61. Yomoda, J., Muraki, M., Kataoka, N., Hosoya, T., Suzuki, M., Hagiwara, M. and Kimura, H. (2008) Combination of Clk family kinase and SRp75 modulates alternative splicing of Adenovirus E1A. Genes Cells, 13(3):233-44.
  62. Ohno, G., Hagiwara, M. and Kuroyanagi, H. (2008) STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo. Genes and Dev., 22(3):360-374.
  63. Kuroyanagi, H, Ohno, G., Mitani, S., and Hagiwara, M. (2007) Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo. Mol. Cell. Biol. 27(24):8612-21.

  64. Ideue T, Sasaki Y, Hagiwara M and Hirose T. (2007) Introns play an essential role in splicing-dependent formation of the exon junction complex. Genes and Dev., 21(16):1993-1998.
  65. Kaida D, Motoyoshi H, Tashiro E, Nojima T, Hagiwara M, Ishigami K, Watanabe H, Kitahara T, Yoshida T, Nakajima H, Tani T, Horinouchi S and Yoshida M (2007) Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA. Nature Chem. Biol., 3(9):576-583.
  66. Nojima,T., Hirose,T.,Kimura,H.,and Hagiwara,M. (2007) The interaction between cap-binding complex and REF is required for intronless mRNA export. J. Biol. Chem. 282(21):15645-15651.
  67. Aida, T., Konishi,A., Hagiwara,M., and Hashimoto,K. (2007) Shortened Life-Spans of Biotech Pioneer Patents in Japan: A Lesson from the DNA Chip. Nature Biotech. 25(5):533-5.
  68. Kimura, H., Takizawa N., Allemand, E., Hori, T., Iborra, F.J., Nozaki, N., Muraki, M., Hagiwara, M.., Krainer, A.R., Fukagawa, T., and Okawa, K. (2006) A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A/H2B. J. Cell Biol. 175(3):389-400.
  69. Kuroyanagi, H., Kobayashi, T., Mitani, S., and Hagiwara, M. (2006) Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo. Nature Methods. 3(11):909-915.
  70. Wada, K., Howard, J.T., McConnell, P., Whitney, O., Lints, T., Rivas, M.V. Horita, H., Patterson, M.A., White, S.A., Scharff, C., Haesler, S., Zhao, S., Sakaguchi, H., Hagiwara, M., Shiraki, T., Hirozane-Kishikawa, T., Skene, P., Hayashizaki, Y., Carninci, P., and Jarvis, E.D. (2006) A molecular neuroethological approach for identifying and characterizing a cascade of behaviorally regulated genes. Proc Natl Acad Sci USA. 103(41):15212-15217
  71. Hirose T., Ideue, T., Nagai, M., Hagiwara, M., Shu, M., and  Steitz, J.A. (2006) A spliceosomal intron-binding protein, IBP160, links position-dependent assembly of intorn-encoded box C/C snoRNP to pre-mRNA splicing. Mol. Cell. 23(5):673-684
  72. Fukuhara T, Hosoya T, Shimizu S, Sumi K, Oshiro T, Yoshinaka Y, Suzuki M, Yamamoto N, Herzenberg LA, Herzenberg LA and Hagiwara M (2006) Utilization of host SR protein kinases and RNA-splicing machinery during viral replication. Proc Natl Acad Sci USA. 103(30):11329-11333
  73. Ohki, N. and Hagiwara, M. (2005) Bio-Object, a stochastic simulator for post-transcriptional regulation. Bioinformatics. 21(10):2478-2487.
  74. Wada, K.,Sakaguchi, H.,Jarvis, E.,and Hagiwara, M. (2004) Differential expression of glutamate receptors in avian neural pathways for learned vocalization. J. Comp. Neurol. 476(1):44-64.
  75. Muraki, M., Ohkawara, B.,Hosoya, T., Onogi, H.,Koizumi, J.,Koizumi, T., Sumi, K., Yomoda, J., Murray, M. V., Kimura, K., Furuichi, K., Shibuya, H., Krainer, A. R., Suzuki, M. and Hagiwara, M. (2004) Manipulation of alternative splicing by a newly developed inhibitor of Clks. J. Biol. Chem. 279(23):24246-24254.
  76. Takano, M., Koyama, Y., Ito, H., Hoshino, S.,Onogi, H.,Hagiwara, M., Furukawa, K. and Horigome,T(2004) Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts. J. Biol. Chem. 279(13):13265-13271.
  77. Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H. and Hagiwara, M. (2004) TNFα-induced ATF3 Expression Is Bidirectionally Regulated by the JNK and ERK Pathways in Vascular Endothelial Cells. Gene to Cells. 9(1):59-70.
  78. Umehara, H., Nishii, Y., Morishima, M., Kakehi, Y., Kioka,N., Amachi,T., Koizumi,J., Hagiwara, M. and Ueda,K (2003) Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear proteinCROP/Luc7A Biochem. Biophys. Res. Comm. 301(2):324-329.
  79. Suzuki,H.,Wu, JH,,Hossain, K.,Ohhata,T., Du, J., Akhand, AA., Hayakawa, A., Kimura,H., Hagiwara ,M., andNakashima,I.(2003) Involvement of MKK6 in TCRa beta(int)CD69(lo):a target population for apoptotic cell death in thymocytes. FASEB J. 17(11):1538-1540.
  80. Katsu, R., Onogi, H., Wada, K., Kawaguchi, K. and Hagiwara, M. (2002) Novel SR-related protein Clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk. J. Biol. Chem. 277(46):44220-44228.
  81. Suzuki, T., Yamakuni , T., Hagiwara, M. , and Ichinose, H. (2002) Identification of ATF-2 as a Transcriptional Regulator for the Tyrosine Hydroxylase Gene. J. Biol. Chem. 277(43):40768-40774.
  82. Kimura Y, Corcoran EE, Eto K, Gengyo-Ando K, Muramatsu MA, Kobayashi R, Freedman JH, Mitani S, Hagiwara M, Means AR, Tokumitsu H. (2002) A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. EMBO Rep. 3(10):962-966.
  83. Wada K, Inoue K, Hagiwara M. (2002) Identification of methylated protein arginine N-methyltransferase 1,PRMT1 with a new expression cloning strategy. Biochem. Biophys. Acta. 1591(1-3):1-10.
  84. Zama T, Aoki R, Kamimoto T, Inoue K, Ikeda Y, Hagiwara M. (2002) A novel dual-specificity phosphatase SKRP1 interacts with the MAPKK MKK7 and inactivates the JNK MAPK pathway: Implication for the precise regulation of the particular MAPK pathway. J. Biol. Chem. 277(26):23909-23918.
  85. Zama T, Aoki R, Kamimoto T, Inoue K, Ikeda Y, Hagiwara M. (2002) Scaffold role of a MAP linase phosphatase, SKRP1 for the JNK signaling pathway. J. Biol. Chem. 277(26):23919-23926.
  86. Kamimoto, T., Zama, T., Aoki, R., Muro, Y. and Hagiwara, M.  (2001) Identification of a novel kinesin-related protein, KRMP1, as a target for mitotic peptidyl-prolyl isomerase Pin1. J. Biol. Chem. 276, 37520-37528.
  87. Kojima, T., Zama, T., Wada, K., Onogi, H. and Hagiwara, M.  (2001) Cloning of Human PRP4 Reveals Interaction with Clk1. J. Biol. Chem. 276, 32247-32256.
  88. Osada, M., Inaba, R., Shinohara, H., Hagiwara, M., Nakamura, M. and Ikawa, Y.  (2001) Regulatory Domain of Protein Stability of Human P51/TAP63, a P53 Homologue. Biochem. and Biophys. Res. Comm. 283, 1135-1141.
  89. Aratani, S., Fujii, R., Oishi, T., Fujita, H., Amano, T., Ohshima, T., Hagiwara, M., Fukamizu, A. and Nakajima, T. (2001) Dual roles of RNA helicase A on CREB-dependent transcription. Mol. Cell. Biol., 21, 4460-4469.
  90. Ueda, H.R., Hagiwara, M. and Kitano, H. (2001) Robust oscillations within the interlocked feedback model of Drosophila circadian rhythm. J. Theoret. Biol. 210, 401-406.
  91. Kuroyanagi, H., Kimura, T., Wada, K., Hisamoto, N., Matsumoto, K. and Hagiwara, M. (2000) SPK-1, a C. elegans SR proteins kinase homologue, is essential for embryogenesis and required for germ line development. Mech. Dev. 99, 51-64.
  92. Muro,Y., Kamimoto,T., Tomita,Y., and Hagiwara, M., (2000) Spectrum of autoantibodies against a dynamin-related protein,dymple. Arthritis Rheumatism 43, 7, 1516-1519.
  93. Inoue, K., Mizuno, T., Wada, K. and Hagiwara, M. (2000) Novel RING finger proteins, Air1p and 2p interact with Hmtlp and inhibit the arginine methylation of Np13p. J. Biol. Chem. 275, 32793-32799.
  94. Usukura, J., Nishizawa, Y., Shimomura, A., Kobayashi, K., Nagatsu, T. and Hagiwara, M. (2000) Direct imaging of phosphorylation dependent congormational change and DNA binding of CREB by electron microscopy. Genes to Cells 5, 515-522.
  95. Nagai, Y., Miyazaki, M., Aoki, R., Zama, T., Inoue, S., Hirose, K., Iino, M. and Hagiwara, M, (2000) A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo. Nat. Biotechnol. 18, 313-316.
  96. Kawahara, K., Watanabe, S., Ohshima, T., Soejima, Y., Oishi, T., Aratani, S., Nakata, M., Shibata, M., Inoue, K., Amano, T., Fujii, R., Yanai, K., Hagiwara, M., Fukamizu, A., Maruyama, I. and Nakajima, T. (1999) Hypernuclear acetylation in atherosclerotic lesions and activated vascular smooth muscle cells. Biochem. Biophys. Res. Comm. 266, 417-424.

  97. Sakaguchi, H., Wada, K., Maekawa, M., Watsuji, T. and Hagiwara, M, (1999) Song-induced phosphorylation of cAMP response element binding protein in the songbird brain. J. Neuroscience 19, 3973-3981.
  98. Koizumi, J., Okamoto, Y., Onogi, H., Mayeda, A., and Krainer, A. and Hagiwara, M, (1999) The subcellular localization of SF2/ASF is regulated by the direct interaction with SR protein kinases (SRPKs). J. Biol. Chem. 274, 11125-11131.
  99. Okamoto, Y., Onogi, H., Honda, R., Yasuda, H., Wakabayashi, T., Nimura, Y. and Hagiwara, M. (1998) cdc2 kinase-mediated phosphorylation of splicing factor SF2/ASF. Biochem. Biophys. Res. Comm. 249, 872-878
  100. Yoshida, K., Imaki, J., Okamoto, Y., Iwakabe, H., Fujisawa, H., Matsuda, A., Nakanishi, S., Matsuda, H. and Hagiwara, M. (1998) CREB-induced transcriptional activation depends on mGluR6 in rod bipolar cells. Mol. Brain Res. 57, 241-247.
  101. Shimomura, A., Okamoto, Y., Hirata, Y., Kobayashi, M., Kawakami, K., Kiuchi, K., Wakabayashi, T. and Hagiwara, M. (1998) Dominant negative ATF1 blocks cyclic AMP-induced neurite outgrowth in PC12D Cells. J. Neurochem. 70, 1029-1034.
  102. Muro, Y., Ogawa, Y., Kato, Y. and Hagiwara, M. (1998) Autoantibody to thioredoxin reductase in an ovarian cancer patient. Biochem. Biophys. Res. Commun. 242, 267-271.
  103. Kuroyanagi, N., Onogi, H., Wakabayashi, T. and Hagiwara, M. (1998) Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles. Biochem. Biophys. Res. Commun. 242, 357-364.
  104. Kamimoto, T., Nagai, Y., Onogi, H., Muro, Y., Wakabayashi, T. and Hagiwara, M. (1998) Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells. J. Biol. Chem. 273,1044-1051
  105. Muro, Y., Kano, T., Sugiura, K. and Hagiwara, M. (1997) Low frequency of autoantibodies against Ki-67 antigen in Japanese patients with systemic autoimmune. J. Autoimmunity 10, 499-503.
  106. Nomoto, S., Watanabe, Y., Ninomiya-Tsuji, J., Yang, L.-X., Kiuchi, K., Hagiwara, M., Hidaka, H., Matsumoto, K. and Irie, K. (1997) Functional analyses of mammalian protein kinase kinase C Isozymes in budding yeast and mammalian fibroblasts. Genes to Cells 2, 601-614.
  107. Nagai, Y., Kojima, T., Muro, Y., Hachiya, T., Nishizawa, Y., Wakabayashi, T. and Hagiwara, M. (1997) Identification of a novel nuclear speckle-type protein, SPOP.  FEBS Lett. 418, 23-26
  108. Masunaga, R., Nagasaka, A., Nakai, A., Kotak, M., Sawai, Y., Oda, N., Mokuno, T., Shimazaki, K., Hayakawa, N., Kato, R., Hiran, E., Hagiwara, M., and Hidaka, H. (1997) Alteration of platelet aggregation in patients with thyroid disorders.  Metabolism 46, 1128-1131.
  109. Watsuji, T., Okamoto, Y., Emi, N., Katsuoka, Y. and Hagiwara. M. (1997) Controlled gene expression with a reverse tetracycline regulated retroviral vector (RTRV) system. Biochem. Biophys. Res. Commun. 234, 769-773
  110. Watanabe, Y., Takaetsu, G., Hagiwara, M., Irie, K. and Matsumoto, K. (1997) Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) Mitogen-activated protein kinase pathway. Mol. Cell. Biol. 17, 2615-2623.
  111. Sugiura, K., Muro, Y., Nagai, Y. and Hagiwara, M. (1997) Expression cloning and intracellular localization of a human ZF5 homologue. Biochem. Biophys. Acta. 1352, 23-26
  112. Muro, Y., Kamimoto, T. and Hagiwara, M. (1997) Anti-Mitosin antibodies in a patient with chronic graft-versus-host disease after allogenic bone marrow transplantation. Bone Marrow Transplantation 19, 951-953.
  113. Kobayashi, M., Shimomura, A., Hagiwara, M. and Kawakami, K. (1997) Phosphorylation of ATF-1 enhances its DNA binding and transcription of the Na, K-ATPase α1 subunit gene promoter. Nucleic Acids Res. 25, 877-882.
  114. Okajima, T., Okuno, S., Fujisawa, H. and Hagiwara, M. (1996) Nuclear localization of Ca2+/Calmodulin-dependent protein kinase IV and calspermin.  Bioimage 4, 117-128.
  115. Moriguchi, T., Toyoshima, F., Gotoh, Y., Iwamatsu, A., Irie, K., Mori, E., Kuroyanagi, N., Hagiwara, M., Matsumoto, K. and Nishida, E. (1996). Purification and identification of a major activator for p38 from osmotically shocked cells: Activation of MAPKK6 by osmotic shock, tumor necrosis factor-a and H2O2.  J. Biol. Chem. 271, 26981-26988. 
  116. Okada, H., Miyamura, K., Ito, T., Hagiwara, M., Wakabayashi, T., Mizuno, M., Colosi, P., Kurtzman, G. and Yoshida, J. (1996) Gene therapy against an experimental glioma using adeno-associated virus vectors. Gene Therapy  3, 957-964.
  117. Shimomura, A., Ogawa, Y., Fujisawa, H. and Hagiwara, M. (1996) Calmodulin-dependent protein kinase II potentiates transcriptional activation through ATF1, but not CREB. J. Biol. Chem. 271, 17957-17960.
  118. Moriguchi, T., Kuroyanagi, N., Yamaguchi, K., Gotoh, Y., Irie, K., Kano, T., Shirakabe, K., Muro, Y., Shibuya, H., Matsumoto, K., Nishida, E. and Hagiwara, M. (1996) A novel kinase cascade mediated by MAPKK6 and MKK3. J. Biol. Chem. 271, 13675-13679.
  119. Yasunami, M., Suzuki, K., Maruyama, H., Kawakami, H., Nagai, Y., Hagiwara, M. and Ohkubo, H. (1996) Molecular cloning and characterization of a cDNA encoding a novel basic helix-loop-helix protein structurally related to NeuroD/BHF1.  Biochem. Biophys. Res. Commun. 220, 754-758.
  120. Okada, H., Yoshida, J., Sokabe, M., Wakabayashi, T. and Hagiwara, M. (1996) Suppression of CD44 expression decreases migration and invasion of human glioma cells. Int. J. Cancer. 66, 255-260
  121. Yoshida, K., Imaki, J., Fujisawa, H., Harada, T., Ohki, K., Matsuda, H. and Hagiwara, M. (1996) Differential distribution of CaM kinases and induction of c-fos expression by flashing and sustained light in rat retinal cells. Invest. Ophthalmol. Vis. Sci. 37, 174-179.
  122. Harada, T., Imaki, J., Hagiwara, M., Ohki, K., Takamura, M., Ohashi, T., Matsuda, H. and Yoshida, K. (1995) Phosphorylation of CREB in rat retinal cells after focal retinal injury. Exp. Eye. Res. 61,769-772
  123. Kano, T., Suzuki, Y., Shibuya, M., Kiuchi, K. and Hagiwara, M. (1995) Cocaine-induced CREB phosphorylation and c-fos expression were suppressed in parkinsonism model mice. NeuroReport  6, 2197-2200.
  124. Ohki, K., Yoshida, K., Hagiwara, M., Harada, T., Takamura, M., Ohashi, T., Matsuda, H. and Imaki, J. (1995) Nitric oxide induces c-fos gene expression via cyclic AMP response element binding protein (CREB) phosphorylation in rat retinal pigment epithelium. Brain Res. 696, 140-144.
  125. Yoshida, K., Imaki, J., Matsuda, H. and Hagiwara, M. (1995) Light-induced CREB phosphorylation and gene expression in rat retinal cells. J. Neurochem. 65,1499-1504.
  126. Okada, H., Yoshida, J., Seo, H., Wakabayashi, T., Sugita K. and Hagiwara, M. (1994). Anti-(glioma surface antigen) monoclonal antibody G-22 recognizes overexpressed CD44 in glioma cells. Cancer Immunol. Immunother. 39, 313-317.
  127. Alberts, A.S., Arias, J., Hagiwara, M., Montminy, R.M. and Feramisco, J.R. (1994) Recombinant cyclic AMP response element binding protein (CREB) phosphorylated on Ser-133 is transcriptionally active upon its introduction into fibroblast nuclei. J. Biol. Chem. 269,7623-7630.
  128. Chrivia, J.C., Kwok, R.P., Lamb, N., Hagiwara, M., Montminy, R.M. and Goodman, R.H. (1993) Phosphorylated CREB binds specifically to the nuclear protein CBP.  Nature 365, 855-859.
  129.  Nakamura, S., Wakusawa, S., Tajima, K., Miyamoto, K.-I., Hagiwara, M. and Hidaka, H. (1993) Effects of isoquinoline sulphonamide compounds on multi-drug-resistant P388 cells. J. Pharmacol. 45, 268-273.
  130. Shimohama, S., Narita, M., Matsushima, H., Kimura, J., Kameyama, M., Hagiwara, M., Hidaka, H. and Taniguchi, T. (1993). Assessment of protein kinase C isozymes by two-site enzyme immunoassay in human brains and changes in Alzheimer’s disease. Neurology 43, 1407-1413.
  131. Hagiwara, M., Brindle, P., Harootunian, A., Armstrong, R., Rivier, J., Vale, W., Tsien, R., and Montminy, R. M. (1993) Stimulus-transcription coupling through the cAMP responsive factor CREB is rate-limited by the nuclear entry of PK-A.  Mol. Cell. Biol. 13, 4852-4859.
  132. Kawamoto, F., Fujioka, H., Murakami, R., Syafruddin, Hagiwara, M., Ishikawa, T. and Hidaka, H. (1993) The roles of Ca2+/calmodulin-andcGMP-dependent pathways in gametogenesis of a rodent malaria parasite, Plasmodium bergheiEur. J. Cell. Biol. 60, 101-107.
  133. Matsushima, H., Shimohama, S., Yamaoka, Y., Kimura, J., Taniguchi, T., Hagiwara, M. and Hidaka, H. (1993) Alteration of human platelet protein kinase C with normal aging. Mech. Ageing Dev. 69,129-36.
  134. Hagiwara, M., Alberts, A., Brindle, P., Meinkoth, J., Feramisco, J., Deng, T., Karin, M., Shenolikar, S. and Montminy, M. (1992) Transcriptional attenuation following cAMP induction requires PP-1-mediated dephosphorylation of CREB. Cell 70, 105-13.
  135. Okazaki, K., Watanabe, M., Ando, Y., Hagiwara, M., Terasawa, M. and Hidaka, H. (1992) Full sequence of neurocalcin, a novel calcium-binding protein abundant in central nervous system. Biochem. Biophys. Res. Commun. 185,147-53.
  136. Goto, S., Kobayashi, M., Murate, T., Hotta, T., Hagiwara, M., Hoshino, T., Hidaka, H. and Saito, H. (1992). Thrombin-induced shape change in human megakaryoblastic leukemic cells, MEG-01, is mediated by protein kinase C. Thromb. Res. 68, 87-95.
  137. Usuda, N., Hagiwara, M., Kong, Y., Shimizu, T., Hidaka, H. and Nagata, T. (1992) Immunohistochemical locazilation of protein kinase C isozymes. Acta. Histochem. Cytochem. 25, 707-715.
  138. Wakusawa, S., Nakamura, S., Tajima, K., Miyamoto, K.-I., Hagiwara, M. and Hidaka, H. (1992) Overcoming of vinblastine resistance by isoquinoline sulfonamide compounds in adriamycin-resistant leukemiacells. Mol. Pharmacol. 41, 1034-1038.
  139. Kato, M., Hagiwara, M. and Hidaka, H. (1992) Identification of a 80 kDa calmodulin-binding protein as a new Ca2+/calmodulin-dependent kinase by renaturation blotting assay (RBA). Biochem. J. 281, 339-42.
  140. Iwamoto, T., Hagiwara, M., Hidaka, H., Isomura, T., Kioussis, D. and Nakashima, I. (1992) Accelerated proliferation and interleukin-2 production of thymocytes by stimulation of soluble anti-CD3 monoclonal antibody in transgenic mice carrying a rabbit protein kinase C alpha. J. Biol. Chem. 267, 18644-18648.
  141. Uchida, C., Hagiwara, M. and Hidaka, H. (1991) Ca(2+)-independent, phospholipid-activated protein kinase in 3Y1 cells. Arch. Biochem. Biophys. 288, 421-426.
  142. Terasawa, M., Hagiwara, M., Hachiya, T., Kobayashi, R., Obata, S., Wakabayashi, T. and Hidaka, H. (1991) Identification and characterization of protein kinase C-related enzymes in frog retina. Arch. Biochem. Biophys. 287, 213-7.
  143. Hagiwara, M., Wakusawa, S., Miyamoto, K., and Hidaka, H. (1991). Obviation of drug resistance and affinity purification of P-glycoprotein by isoquinoline sulfonamides. Cancer Lett. 60, 103-7.
  144. Sumi, M., Kiuchi, K., Ishikawa, T., Ishii, A., Hagiwara, M., Nagatsu, T. and Hidaka, H. (1991). The newly synthesized selective Ca2+/calmodulin dependent protein kinase II inhibitor KN-93 reduces dopamine contentsin PC12h cells.  Biochem. Biophys. Res. Commun. 181, 968-975.
  145. Shimizu, T., Usuda, N., Sugenoya, A., Masuda, H., Hagiwara, M., Hidaka, H., Nagata, T. and Iida, F. (1991) Immunohistochemical evidence for the overexpression of protein kinase C in proliferative diseases of human thyroid.  Cell. Mol. Biol. 37, 813-821.
  146. Usuda, N., Kong, Y., Hagiwara, M., Uchida, C., Terasawa, M., Nagata, T. and Hidaka, H. (1991) Differential localization of proteikinase C isozymes in retinal neurons. J. Cell Biol. 112, 1241-1247.
  147. Kato, M., Hagiwara, M., Nimura, Y., Shionoya, S. and Hidaka, H. (1991). Purification and characterization of calcium-calmodulin kinase II from human parathyroid glands. J Endocrinol. 131, 155-162.
  148. Koyama, Y., Fukaya, Y., Ueda, H., Hachiya, T., Hagiwara, M., Hidaka, H. and Marunouchi, T. (1990). Expression of protein kinase C isozyme in human Langerhans’ cells. Acta. Derm. Venereol. Stockh. 70, 502-505.
  149. Koyama, Y., Hachiya, T., Hagiwara, M., Kobayashi, M., Ohashi, K., Hoshino, T., Hidaka, H. and Marunouchi, T. (1990). Expression of kinaseC isozyme in epidermal Langerhans cells of the mouse. J. Invest. Dermatol. 94, 677-680.
  150. Onoda, K., Hagiwara, M., Hachiya, T., Usuda, N., Nagata, T. and Hidaka, H. (1990) Different expression of protein kinase C isozymes inpancreatic islet cells.  Endocrinology 126, 1235-40.
  151. Chijiwa, T., Mishima, A., Hagiwara, M., Sano, M., Hayashi, K., Inoue,T., Naito, K., Toshioka, T. and Hidaka, H. (1990) Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide, of PC12D pheochromocytoma cells. J. Biol. Chem. 265, 5267-5272.
  152. Miyamoto, K., Wakusawa, S., Nakamura, S., Koshiura, R., Otsuka, K., Naito, K., Hagiwara, M. and Hidaka, H. (1990) Circumvention of multi-drug resistance in P388 murine leukemia cells by a novel inhibitor of cyclic AMP-dependent protein kinase, H-87. Cancer Lett. 51, 37-42.
  153. Hachiya, T., Hagiwara, M. and Hidaka, H. (1990) Immunoaffinity purification of type I protein kinase C. Arch. Biochem. Biophys. 280, 358-361.
  154. Sumi, M., Hagiwara, M. and Hidaka, H. (1990) δ-Indobufen inhibits collagen-induced intracellular calcium mobilization and inositol phosphates formation in human platelets. Thromb. Res. 59,319-326.
  155. Shimosawa, S., Hachiya, T., Hagiwara, M., Usuda, N., Sugita, K. and Hidaka, H. (1990) Type-specific expression of protein kinase C isozymes in CNS tumor cells.  Neurosci. Lett. 108, 11-16.
  156. Hagiwara, M., Uchida, C., Usuda, N., Nagata, T., and Hidaka, H. (1990) Zeta-related protein kinase C in nuclei of nerve cells. Biochem. Biophys. Res. Commun. 168, 161-168.
  157. Hagiwara, M., Hachiya, T., Watanabe, M., Usuda, N., Iida, F., Tamai, K. and Hidaka, H. (1990) Assessment of protein kinase C isozymes by enzyme immunoassay and overexpression of type II in thyroid adenocarcinoma. Cancer Res. 50, 5515-5519.
  158. Hagiwara, M., Sumi, M., Usuda, N., Nagata, T. and Hidaka, H. (1990) Discrepancy of protein kinase C translocation and platelet aggregation-immunocytobiochemical evidence. Arch. Biochem. Biophys. 280, 201-5
  159. Tokumitsu, H., Chijiwa, T., Hagiwara, M., Mizutani, A., Terasawa, M. and Hidaka, H. (1990). KN-62, 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine, a specific inhibitor of Ca2+/calmodulin-dependent protein kinase II. J. Biol. Chem. 265, 4315-4320
  160. Watanabe, M., Hachiya, T., Hagiwara, M. and Hidaka, H. (1989) Identification of type III protein kinase C in bovine aortic tissue. Arch. Biochem. Biophys. 273, 165-169.
  161. Mamiya, S., Hagiwara, M., Ishikawa, T. and Hidaka, H. (1989) Specific inhibition of 40 K protein phosphorylation in platelet by a new antithrombotic agent, δ-indobufen. Thromb. Res. 54, 447-456.
  162. Mamiya, S., Hagiwara, M., Inoue, S. and Hidaka, H. (1989) Thyroid hormones inhibit platelet function and myosin light chain kinase. J. Biol. Chem. 264, 8575-8279.
  163. Kariya, K., Kawahara, Y., Fukuzaki, H., Hagiwara, M., Hidaka, H., Fukumoto, Y. and Takai, Y. (1989). Two types of protein kinase C With different functions in cultured rabbit aortic smooth muscle cells. Biochem. Biophys. Res. Commun. 161, 1020-1027.
  164. Tokumitsu, H., Hagiwara, M., Onoda, K. and Hidaka, H. (1989) Anti-gizzard MLCK monoclonal antibody MM13 inhibits superprecipitation and phosphorylation of bovine aortic smooth muscle actomyosin. J. Biochem. 106, 511-514.
  165. Chijiwa, T., Hagiwara, M. and Hidaka, H. (1989) A newly synthesized selective casein kinase I inhibitor, N-(2-aminoethy1)-5-chloroisoquinoline-8-sulfonamide,and affinity purification of casein kinase C in neuroblastoma NBC-20 cell: dependence on 1,2-diacylglycerol content and free calcium. J Biol Chem. 264, 5267-5272.
  166. Fu, T., Okano, Y., Hagiwara, M., Hidaka, H. and Nozawa, Y. (1989) NCB-20 cell: dependence on 1,2-diacylglycerol content and free calcium. Biochem. Biophys. Res. Commun. 162, 1279-1286.
  167. Hagiwara, M., Tokumitsu, H., Onoda, K., Tanaka, T., Ito, M., Kato, N. and Hidaka, H. (1989) Monoclonal antibody assessment of tissue- and species- specific myosin light chain kinase isozymes. J. Biochem. 106, 71-75.
  168. Hagiwara, M., Mamiya, S. and Hidaka, H. (1989) Selective binding of L-thyroxine by myosin light chain kinase. J. Biol. Chem. 264, 40-44.
  169. Ishikawa, T., Chijiwa, T., Hagiwara, M., Mamiya, S. and Hidaka, H. (1989) Thyroid hormones directly interact with vascular smooth muscle strips. Mol. Pharmacol. 35, 760-765.
  170. Tanaka, T., Hagiwara, M., Hidaka, H., Nunoki, K., Ohta, H., Onoda, K., Ito, M., Ohkubo, S., Hiai, H. and Nishizuka, Y. (1989) Selective low level of protein kinase C isozyme in a tumor promoter-dependent mouse leukemia cell line. Biochem. Biophys. Res. Commun. 164,1397-1401.
  171. Abougou, J. C., Hagiwara, M., Hachiya, T., Terasawa, M., Hidaka, H. and Hartshorne, D. J. (1989) Phosphorylation of caldesmon. FEBS Lett. 257, 408-410.
  172. Hagiwara, M., Mamiya, S., Ochiai, M. and Hidaka, H. (1988) Thyroid hormones inhibit the Ca2+ calmodulin-induced activation of myosin light chain kinase. Biochem. Biophys. Res. Commun. 152, 270-276.
  173. Hagiwara, M., Inagaki, M., Takahashi, J., Yoshida, T. and Hidaka, H. (1988) A fluorescent protein kinase C inhibitor: 1-(1-hydroxy-5-isoquinolinylsulfonyl) piperazine. Pharmacology 36, 365-370.
  174. Ishikawa, T., Chijiwa, T., Hagiwara, M., Mamiya, S., Saitoh, M. and Hidaka, H. (1988) ML-9 inhibits the vascular contraction via the inhibition of myosin light chain phosphorylation. Mol. Pharmacol. 33, 598-603.
  175. Hagiwara, M., Inagaki, M., Kanamura, K., Ohta, H. and Hidaka, H. (1988) Inhibitory effects of aminoglycosides on renal protein phosphorylation by protein kinase C. J. Pharmacol. Exp. Ther. 244, 355-360.
  176. Kiuchi, K., Hagiwara, M., Hidaka, H. and Nagatsu, T. (1988) Effect of the 1-methyl-4-phenylpyridinium ion on phosphorylation of tyrosine hydroxylase in rat pheochromocytoma PC12h cells. Neurosci. Lett. 89, 209-215.
  177. Ito, T., Tanaka, T., Yoshida, T., Onoda, K., Ohta, H., Hagiwara, M., Itoh, Y., Ogura, M., Saito, H. and Hidaka, H. (1988) Immunocytochemical evidence for translocation of protein kinase C in human megakaryoblastic leukemic cells: synergistic effects of Ca2+ and activators of protein kinase C on the plasma membrane association. J. Cell Biol. 107, 929-937.
  178. Hagiwara, M., Ochiai, M., Owada, K., Tanaka, T. and Hidaka, H. (1988) Modulation of tyrosine phosphorylation of p36 and other substrates by the S-100 protein. J. Biol. Chem. 263, 6438-6441.
  179. Hagiwara, M., Inoue, S., Tanaka, T., Nunoki, K., Ito, M. and Hidaka, H. (1988) Differential effects of flavonoids as inhibitors of tyrosine protein kinases and serine/threonine protein kinases. Biochem. Pharmacol. 37, 2987-2992.
  180. Tanaka, T., Ishikawa, T., Hagiwara, M., Onoda, K., Itoh, H. and Hidaka, H. (1988) Effects of cilostazol, a selective cAMP phosphodiesterase inhibitor on the contraction of vascular smooth muscle. Pharmacology 36, 313-320.
  181. Hidaka, H., Tanaka, T., Onoda, K., Hagiwara, M., Watanabe, M., Ohta, H., Ito, Y., Tsurudome, M. and Yoshida, T. (1988). Cell type-specific expression of protein kinase C isozymes in the rabbit cerebellum. J. Biol. Chem. 263, 4523-4526.
  182. Watanabe, M., Hagiwara, M., Onoda, K. and Hidaka, H. (1988). Monoclonal antibody recognition of two subtype forms of protein kinase C in human platelets.  Biochem. Biophys. Res. Commun. 152, 642-648.
  183. Hagiwara, M., Inagaki, M., Watanabe, M., Ito, M., Onoda, K., Tanaka, T. and Hidaka, H. (1987) Selective modulation of calcium-dependent myosin phosphorylation by novel protein kinase inhibitors, isoquinoline sulfonamide derivatives. Mol. Pharmacol. 32, 7-12.
  184. Hagiwara, M., Inagaki, M. and Hidaka, H. (1987) Specific binding of a novel compound, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide to the active site of cAMP-dependent protein kinase. Mol. Pharmacol. 31, 523-528.
  185. Inagaki, M., Hagiwara, M., Saitoh, M. and Hidaka, H. (1986) Protein kinase C negatively modulated by phorbol ester.  FEBS. Lett. 202, 277-281.
  186. Inagaki, M., Kawamoto, S., Itoh, H., Saitoh, M., Hagiwara, M., Takahashi, J. and Hidaka, H. (1986) Naphthalenesulfonamides as calmodulin antagonists and protein kinase inhibitors. Mol. Pharmacol. 29, 577-81.
  187. Hagiwara, M., Endo, T., Kanayama, T. and Hidaka, H. (1984) Effect of (3-chloroanilino)-4-phenylphthalazine (MY-5445), a specific inhibitor of cyclic GMP phosphodiesterase, on human platelet aggregation. J. Pharmacol. Exp. Ther. 228, 467-471.
  188. Hagiwara, M., Endo, T. and Hidaka, H. (1984) Effects of vinpocetine on cyclic nucleotide metabolism in vascular smooth muscle. Biochem. Pharmacol. 33, 453-457.