Akihide Takeuchi

AFFILIATION : Dept. of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Yoshidakonoe, Sakyo-ku, Kyoto 606-8501, Japan

Degree : M.D. , Ph.D.

Tel : +81-75-753-4670,  Fax: +81-75-751-7529 

E-MAIL :  takeuchi.akihide.8r<a>kyoto-u.ac.jp

 

RESEARCH & PROFESSIONAL EXPERIENCE

Akihide Takeuchi MD., PhD. is an associate professor of Department of Anatomy and Developmental Biology at Graduate School of Medicine, Kyoto University. Takeuchi has a MD in Nagoya University School of Medicine, Nagoya, Japan, obtained the medical license, and had training as a neurosurgeon for several years. Takeuchi has a Ph.D. in Immunology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Takeuchi’s research interest is in deciphering the molecular mechanism of brain development through understanding gene regulation. Takeuchi got post-doctoral training in National Center for Geriatrics and Gerontology National Center for Geriatrics and Gerontology, Obu, Aichi, Japan. There he found essential transcription factor that regulates the development of germ cells and neurogenesis by generating gene targeting mouse (Takeuchi et al, , Nat Genet 2003). To further deeply learn about neuronal development, he got training at the Salk Institute for Biological Studies, La Jolla, California U.S.A. under the supervision of Dr. Dennis DM O’Leary. These he found essential regulation of corticogenesis (Takeuchi and O’Leary, J Neurosci 2006, Takeuchi et al, Cereb Cortex 2006).

From 2006, Takeuchi joined to Dr. Hagiwara’s lab and focused on deciphering the regulatory mechanism of gene expression including mRNA regulation. For this purpose, he developed mammalian splicing reporter system that enables real time visualization of mRNA regulation in vivo (Takeuchi et al, PLoS One 2010). This system is quite useful for analyzing the regulatory mechanism of mRNA metabolism in normal also disease conditions caused by genetic mutations, thus he patented with Dr. Hagiwara in Japan, US and Europe. Splicing reporter system was applied to develop novel therapeutic method manipulating mRNA regulation with small chemicals to cure genetic diseases (Yoshida et al. PNAS 2015, and other RNA diseases are currently on going).

Takeuchi also analyzing the functions of RNA-binding proteins (RBPs) those are essential for neuronal or muscular development. He identified several cell-type specific RBPs and analyzed their functions from in vitro to in vivo using CLIP for the purposes of large-scale mapping of protein-RNA interactions and also gene targeting mice for phenotypic analyses. From combining comprehensive analyses, he revealed that RNA-binding proteins play essential roles in neuronal development or muscle growth through regulating the transcriptome of functional gene clusters (Takeuchi et al, Cell Reports 2018, Hosokawa et al, iScience 2019).

 

Selected publications

1  Hosokawa, M., Takeuchi, A. (corresponding author), Tanihata, J., Iida, K., Takeda, S. & Hagiwara, M. Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy. iScience (Cell Press) 13, 229-242, 2019

2  Takeuchi, A. (corresponding author), Iida, K., Tsubota, T., Hosokawa, M., Denawa, M., Brown, J. B., Ninomiya, K., Ito, M., Kimura, H., Abe, T., Kiyonari, H., Ohno, K. & Hagiwara, M. Loss of Sfpq Causes Long-Gene Transcriptopathy in the Brain. Cell Rep 23, 1326-1341, 2018

3  Takeuchi, A., Hosokawa, M., Nojima, T. & Hagiwara, M. Splicing reporter mice revealed the evolutionally conserved switching mechanism of tissue-specific alternative exon selection. PLoS One 5, e10946, 2010

4  Takeuchi, A., Hamasaki, T., Litwack, E. D. & O’Leary, D. D. Novel IgCAM, MDGA1, expressed in unique cortical area- and layer-specific patterns and transiently by distinct forebrain populations of Cajal-Retzius neurons. Cereb. Cortex 17, 1531-1541, 2007

5  Takeuchi, A. & O’Leary, D. D. Radial migration of superficial layer cortical neurons controlled by novel Ig cell adhesion molecule MDGA1. J. Neurosci. 26, 4460-4464, 2006

6  Takeuchi, A., Mishina, Y., Miyaishi, O., Kojima, E., Hasegawa, T. & Isobe, K. Heterozygosity with respect to Zfp148 causes complete loss of fetal germ cells during mouse embryogenesis. Nat. Genet. 33, 172-176, 2003

7   Kojima, E., Takeuchi, A., Haneda, M., Yagi, A., Hasegawa, T., Yamaki, K., Takeda, K., Akira, S., Shimokata, K. & Isobe, K. The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress: elucidation by GADD34-deficient mice. FASEB J. 17, 1573-1575, 2003

8   Takeuchi, A., Isobe, K. I., Miyaishi, O., Sawada, M., Fan, Z. H., Nakashima, I. & Kiuchi, K. Microglial NO induces delayed neuronal death following acute injury in the striatum. Eur. J. Neurosci. 10, 1613-1620, 1998